Article
Effect of Asp122 Mutation on the Hydride Transfer in E. coli DHFR Demonstrates the Goldilocks of Enzyme Flexibility.
The journal of physical chemistry. B - 23 Aug 2018
Mhashal Anil R, Pshetitsky Yaron, Eitan Reuven, Cheatum Christopher M, Kohen Amnon, Major Dan Thomas
Abstract excerpt
Dihydrofolate reductase (DHFR) catalyzes the reduction of dihydrofolate (DHF) to tetrahydrofolate (THF) in the presence of NADPH. The key hydride transfer step in the reaction is facilitated by a combination of enzyme active site preorganization and correlated protein motions in the Michaelis-Menten (E:NADPH:DHF) complex. The present theoretical study employs mutagenesis to examine the relation between structural...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.