Article
Generation and primary characterization of iAM-1, a versatile new line of conditionally immortalized atrial myocytes with preserved cardiomyogenic differentiation capacity.
Cardiovascular research - 1 Dec 2018
Liu Jia, Volkers Linda, Jangsangthong Wanchana, Bart Cindy I, Engels Marc C, Zhou Guangqian, Schalij Martin J, Ypey Dirk L, Pijnappels Daniël A, de Vries Antoine A F
Abstract excerpt
Aims: The generation of homogeneous cardiomyocyte populations from fresh tissue or stem cells is laborious and costly. A potential solution to this problem would be to establish lines of immortalized cardiomyocytes. However, as proliferation and (terminal) differentiation of cardiomyocytes are mutually exclusive processes, their permanent immortalization causes loss of electrical and mechanical functions. We...
Topics
- Animals
- Animals, Newborn
- Atrial Fibrillation
- Cell Differentiation
- Cell Line, Transformed
- Cell Proliferation
- Gene Expression Regulation
- Heart Atria
- Heart Rate
- Membrane Potentials
- Muscle Development
- Myocytes, Cardiac