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Article

Effects of active site residues of 3α-hydroxysteroid dehydrogenase from pseudomonas sp. b-0831 on its catalysis and cofactor binding.

Bioscience, biotechnology, and biochemistry - 1 Oct 2018

Shiota Ayako, Inaba Satomi, Oda Masayuki

Abstract excerpt

We overexpressed and purified 3α-hydroxysteroid dehydrogenase from Pseudomonas sp. B-0831 (Ps3αHSD) and its mutants where the active site residues known as the SYK triad, Ser114, Tyr153, and Lys157, were mutated. Ps3αHSD catalyzes the reaction by using a nucleotide cofactor. The NADH binding affinity of K157A mutant was much lower than that of the wild-type, mainly due to loss of a hydrogen bond. The decreased...

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PMID: 29912635
DOI: 10.1080/09168451.2018.1486175

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SYK

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