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Nanopore sequencing of drug-resistance-associated genes in malaria parasites, Plasmodium falciparum.

Scientific reports - 29 May 2018

Runtuwene Lucky R, Tuda Josef S B, Mongan Arthur E, Makalowski Wojciech, Frith Martin C, Imwong Mallika, Srisutham Suttipat, Nguyen Thi Lan Anh, Tuan Nghia Nguyen, Eshita Yuki, Maeda Ryuichiro, Yamagishi Junya, Suzuki Yutaka

Abstract excerpt

Here, we report the application of a portable sequencer, MinION, for genotyping the malaria parasite Plasmodium falciparum. In the present study, an amplicon mixture of nine representative genes causing resistance to anti-malaria drugs is diagnosed. First, we developed the procedure for four laboratory strains (3D7, Dd2, 7G8, and K1), and then applied the developed procedure to ten clinical samples. We sequenced...

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Identifiers

PMID: 29844487
DOI: 10.1038/s41598-018-26334-3
PMCID: PMC5974085

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