Article
High-Throughput Screening Enhances Kidney Organoid Differentiation from Human Pluripotent Stem Cells and Enables Automated Multidimensional Phenotyping.
Cell stem cell - 1 Jun 2018
Czerniecki Stefan M, Cruz Nelly M, Harder Jennifer L, Menon Rajasree, Annis James, Otto Edgar A, Gulieva Ramila E, Islas Laura V, Kim Yong Kyun, Tran Linh M, Martins Timothy J, Pippin Jeffrey W, Fu Hongxia, Kretzler Matthias, Shankland Stuart J, Himmelfarb Jonathan, Moon Randall T, Paragas Neal, Freedman Benjamin S
Abstract excerpt
Organoids derived from human pluripotent stem cells are a potentially powerful tool for high-throughput screening (HTS), but the complexity of organoid cultures poses a significant challenge for miniaturization and automation. Here, we present a fully automated, HTS-compatible platform for enhanced differentiation and phenotyping of human kidney organoids. The entire 21-day protocol, from plating to...
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