Article
Facilitated Protein Association via Engineered Target Search Pathways Visualized by Paramagnetic NMR Spectroscopy.
Structure (London, England : 1993) - 5 Jun 2018
An So Young, Kim Eun-Hee, Suh Jeong-Yong
Abstract excerpt
Proteins assemble to form functional complexes via the progressive evolution of nonspecific complexes formed by transient encounters. This target search process generally involves multiple routes that lead the initial encounters to the final complex. In this study, we have employed NMR paramagnetic relaxation enhancement to visualize the encounter complexes between histidine-containing phosphocarrier protein and...
Topics
- Bacterial Proteins
- Binding Sites
- Escherichia coli Proteins
- Genetic Engineering
- Kinetics
- Models, Molecular
- Multiprotein Complexes
- Mutation
- Nuclear Magnetic Resonance, Biomolecular
- Peptide Fragments
- Phosphoenolpyruvate Sugar Phosphotransferase System
- Protein Binding
- Protein Conformation