Article
Molecular procedure for rapid detection of Burkholderia mallei and Burkholderia pseudomallei.
Journal of clinical microbiology - 1 Sept 1998
Bauernfeind A, Roller C, Meyer D, Jungwirth R, Schneider I
Abstract excerpt
A PCR procedure for the discrimination of Burkholderia mallei and Burkholderia pseudomallei was developed. It is based on the nucleotide difference T 2143 C (T versus C at position 2143) between B. mallei and B. pseudomallei detected in the 23S rDNA sequences. In comparison with conventional meth...
Topics
- Base Sequence
- Burkholderia
- Burkholderia Infections
- Burkholderia pseudomallei
- DNA Primers
- DNA, Ribosomal
- Electrophoresis, Agar Gel
- Genetic Variation
- Humans
- Medical Laboratory Personnel
- Melioidosis
- Molecular Sequence Data
- Nucleic Acid Conformation
- Occupational Diseases
- Polymerase Chain Reaction
- RNA, Ribosomal, 23S
- Sensitivity and Specificity
- Sequence Alignment