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Article

BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei.

PloS one - 12 Nov 2010

Bowers Jolene R, Engelthaler David M, Ginther Jennifer L, Pearson Talima, Peacock Sharon J, Tuanyok Apichai, Wagner David M, Currie Bart J, Keim Paul S

Abstract excerpt

A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional...

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Identifiers

PMID: 21103048
DOI: 10.1371/journal.pone.0015413
PMCID: PMC2980470

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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