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Article

Introducing structure-switching functionality into small-molecule-binding aptamers via nuclease-directed truncation.

Nucleic acids research - 27 Jul 2018

Wang Zongwen, Yu Haixiang, Canoura Juan, Liu Yingzhu, Alkhamis Obtin, Fu Fengfu, Xiao Yi

Abstract excerpt

We report a broadly applicable enzyme digestion strategy for introducing structure-switching functionality into small-molecule-binding aptamers. This procedure is based on our discovery that exonuclease III (Exo III) digestion of aptamers is greatly inhibited by target binding. As a demonstration, we perform Exo III digestion of a pre-folded three-way-junction (TWJ)-structured cocaine-binding aptamer and a...

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Identifiers

PMID: 29718419
DOI: 10.1093/nar/gky305
PMCID: PMC6061857

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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