Article
Field-deployable viral diagnostics using CRISPR-Cas13.
Science (New York, N.Y.) - 27 Apr 2018
Myhrvold Cameron, Freije Catherine A, Gootenberg Jonathan S, Abudayyeh Omar O, Metsky Hayden C, Durbin Ann F, Kellner Max J, Tan Amanda L, Paul Lauren M, Parham Leda A, Garcia Kimberly F, Barnes Kayla G, Chak Bridget, Mondini Adriano, Nogueira Mauricio L, Isern Sharon, Michael Scott F, Lorenzana Ivette, Yozwiak Nathan L, MacInnis Bronwyn L, Bosch Irene, Gehrke Lee, Zhang Feng, Sabeti Pardis C
Abstract excerpt
Mitigating global infectious disease requires diagnostic tools that are sensitive, specific, and rapidly field deployable. In this study, we demonstrate that the Cas13-based SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) platform can detect Zika virus (ZIKV) and dengue virus (DENV) in patient samples at concentrations as low as 1 copy per microliter. We developed HUDSON (heating unextracted...
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