Article
Mutations in the Non-Structural Protein-Coding Sequence of Protoparvovirus H-1PV Enhance the Fitness of the Virus and Show Key Benefits Regarding the Transduction Efficiency of Derived Vectors.
Viruses - 27 Mar 2018
Hashemi Hamidreza, Condurat Alexandra-Larisa, Stroh-Dege Alexandra, Weiss Nadine, Geiss Carsten, Pilet Jill, Cornet Bartolomé Carles, Rommelaere Jean, Salomé Nathalie, Dinsart Christiane
Abstract excerpt
Single nucleotide changes were introduced into the non-structural (NS) coding sequence of the H-1 parvovirus (PV) infectious molecular clone and the corresponding virus stocks produced, thereby generating H1-PM-I, H1-PM-II, H1-PM-III, and H1-DM. The effects of the mutations on viral fitness were analyzed. Because of the overlapping sequences of NS1 and NS2, the mutations affected either NS2 (H1-PM-II, -III) or...
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