Article
Dot-blot-SNP analysis for practical plant breeding and cultivar identification in rice.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 Jun 2006
Shirasawa K, Shiokai S, Yamaguchi M, Kishitani S, Nishio T
Abstract excerpt
We report dot-blot hybridization with allele-specific oligonucleotides for single nucleotide polymorphisms (SNPs) analysis to be applicable for practical plant breeding and cultivar identification. Competitive hybridization of a digoxigenin-labeled oligonucleotide having the sequence of a mutant allele (or a wild-type allele) together with an unlabeled oligonucleotide having the sequence of a wild-type allele (or...
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