Article
Comparison of reduced metagenome and 16S rRNA gene sequencing for determination of genetic diversity and mother-child overlap of the gut associated microbiota.
Journal of microbiological methods - 1 Jun 2018
Ravi Anuradha, Avershina Ekaterina, Angell Inga Leena, Ludvigsen Jane, Manohar Prasanth, Padmanaban Sumathi, Nachimuthu Ramesh, Snipen Lars, Rudi Knut
Abstract excerpt
Use of the 16S rRNA gene in microbiota studies is limited by the lack of taxonomic and functional resolution. High resolution analyses are particularly important for understanding transmission and persistence of bacteria. The aim of our work was therefore to compare a novel reduced metagenome sequencing (RMS) approach with 16S rRNA gene sequencing to determine both the metagenome genetic diversity and the...
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