Article
Designing, optimization, and validation of whole blood direct T-ARMS PCR for precise and rapid genotyping of complex vertebral malformation in cattle.
BMC biotechnology - 22 May 2021
Alyethodi R R, Singh U, Kumar S, Alex R, Sengar G S, Raja T V, Deb R, Prakash B
Abstract excerpt
BACKGROUND: DNA testing in the cattle industry undergoes multiple hurdles. Successful genotyping involves the transportation of samples from the field to the laboratory in a chilled environment followed by DNA extraction, and finally, a specific genotyping protocol is followed. Various researches are focused on overcoming these issues. Microcards offer blood transportation at ambient temperature. Direct PCR...
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