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Article

Analyzing Protein Domain Interactions in Chemoreceptors by In Vivo PEGylation.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018

Watts Kylie J, Johnson Mark S

Abstract excerpt

The instability of some proteins can hamper in vitro studies. This is true for the membrane-bound aerotaxis receptor, Aer, which exhibits significant proteolysis during the preparation of membrane vesicles. Permeabilized cells can closely mimic in vivo conditions, maintaining the intracellular milieu and geometry of interacting domains. Here, we describe an optimized method for determining solvent accessibility...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 29429089
DOI: 10.1007/978-1-4939-7577-8_13

Genes

HAMP
SDS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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