Article
SP174 Antibody Lacks Specificity for NRAS Q61R and Cross-Reacts With HRAS and KRAS Q61R Mutant Proteins in Malignant Melanoma.
Applied immunohistochemistry & molecular morphology : AIMM - 1 Jan 2018
Felisiak-Goląbek Anna, Inaguma Shingo, Kowalik Artur, Wasąg Bartosz, Wang Zeng-Feng, Zięba Sebastian, Pięciak Liliana, Ryś Janusz, Kopczynski Janusz, Sarlomo-Rikala Maarit, Góźdź Stanislaw, Lasota Jerzy, Miettinen Markku
Abstract excerpt
HRAS, KRAS, and NRAS, highly homologous proteins, are often mutationally activated in cancer. Usually, mutations cluster in codons 12, 13, and 61 and are detected by molecular genetic testing of tumor DNA. Recently, immunohistochemistry with SP174 antibody has been introduced to detect NRAS Q61R-mutant protein. Studies on malignant melanomas showed that such an approach could be a viable alternative to molecular...
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