Article
Application of directed evolution to develop ethanol tolerant Oenococcus oeni for more efficient malolactic fermentation.
Applied microbiology and biotechnology - 1 Jan 2018
Betteridge Alice L, Sumby Krista M, Sundstrom Joanna F, Grbin Paul R, Jiranek Vladimir
Abstract excerpt
Malolactic fermentation (MLF) is an important step in winemaking, which can be notoriously unreliable due to the fastidious nature of Oenococcus oeni. This study aimed to use directed evolution (DE) to produce a more robust strain of O. oeni having the ability to withstand high ethanol concentrations. DE involves an organism mutating and potentially adapting to a high stress environment over the course of...
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