Article
[A bioinformatic pipeline for NGS data analysis and mutation calling in human solid tumors].
Biomeditsinskaia khimiia - 1 Oct 2017
Tsukanov K Yu, Krasnenko A Yu, Plakhina D A, Korostin D O, Churov A V, Druzhilovskaya O S, Rebrikov D V, Ilinsky V V
Abstract excerpt
We aimed to develop a pipeline for the bioinformatic analysis and interpretation of NGS data and detection of a wide range of single-nucleotide somatic mutations within tumor DNA. Initially, the NGS reads were submitted to a quality control check by the Cutadapt program. Low-quality 3¢-nucleotides were removed. After that the reads were mapped to the reference genome hg19 (GRCh37.p13) by BWA. The SAMtools program...
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