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Article

Mutations of Residues in Pocket P1 of a Cyclodipeptide Synthase Strongly Increase Product Formation.

Journal of natural products - 22 Nov 2017

Brockmeyer Kirsten, Li Shu-Ming

Abstract excerpt

Expression of a cyclodipeptide synthase gene from Nocardiopsis prasina (CDPS-Np) in Escherichia coli resulted in the formation of cyclo-(l-Tyr-l-Tyr) (1) as the minor and cyclo-(l-Tyr-l-Phe) (2) as the major products. Site-directed mutagenesis revealed a strong influence on product accumulation of the amino acid residues in pocket P1. An 8-fold increase in product formation for 1 and 10-fold for 2 were detected...

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Identifiers

PMID: 29064250
DOI: 10.1021/acs.jnatprod.7b00430

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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