Article
Enzyme engineering: reaching the maximal catalytic efficiency peak.
Current opinion in structural biology - 1 Dec 2017
Goldsmith Moshe, Tawfik Dan S
Abstract excerpt
The practical need for highly efficient enzymes presents new challenges in enzyme engineering, in particular, the need to improve catalytic turnover (kcat) or efficiency (kcat/KM) by several orders of magnitude. However, optimizing catalysis demands navigation through complex and rugged fitness landscapes, with optimization trajectories often leading to strong diminishing returns and dead-ends. When no further...
Topics
- Catalysis
- Enzyme Activation
- Enzymes
- Epistasis, Genetic
- Evolution, Molecular
- Genetic Fitness
- Mutation
- Protein Engineering
- Protein Stability