Genome-wide engineering of an infectious clone of herpes simplex virus type 1 using synthetic genomics assembly methods

Abstract excerpt

, replicated with kinetics similar to wild-type virus. We demonstrated the utility of this modular assembly technology by making numerous modifications to a single gene, making changes to two genes at the same time and, finally, generating individual and combinatorial deletions to a set of five conserved genes that encode virion structural proteins. While the ability to perform genome-wide editing through...

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