Article
In vivo chlorophyll fluorescence screening allows the isolation of a Chlamydomonas mutant defective for NDUFAF3, an assembly factor involved in mitochondrial complex I assembly.
The Plant journal : for cell and molecular biology - 1 Nov 2017
Massoz Simon, Hanikenne Marc, Bailleul Benjamin, Coosemans Nadine, Radoux Michèle, Miranda-Astudillo Hector, Cardol Pierre, Larosa Véronique, Remacle Claire
Abstract excerpt
The qualitative screening method used to select complex I mutants in the microalga Chlamydomonas, based on reduced growth under heterotrophic conditions, is not suitable for high-throughput screening. In order to develop a fast screening method based on measurements of chlorophyll fluorescence, we first demonstrated that complex I mutants displayed decreased photosystem II efficiency in the genetic background of...
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