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Article

A forward genetic screen identifies mutants deficient for mitochondrial complex I assembly in Chlamydomonas reinhardtii.

Genetics - 1 Jun 2011

Barbieri M Rosario, Larosa Véronique, Nouet Cécile, Subrahmanian Nitya, Remacle Claire, Hamel Patrice P

Abstract excerpt

Mitochondrial complex I is the largest multimeric enzyme of the respiratory chain. The lack of a model system with facile genetics has limited the molecular dissection of complex I assembly. Using Chlamydomonas reinhardtii as an experimental system to screen for complex I defects, we isolated, via forward genetics, amc1-7 nuclear mutants (for assembly of mitochondrial complex I) displaying reduced or no complex I...

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Identifiers

PMID: 21467570
DOI: 10.1534/genetics.111.128827
PMCID: PMC3122308

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