Article
Large-scale insertional mutagenesis of Chlamydomonas supports phylogenomic functional prediction of photosynthetic genes and analysis of classical acetate-requiring mutants.
The Plant journal : for cell and molecular biology - 1 Apr 2015
Dent Rachel M, Sharifi Marina N, Malnoë Alizée, Haglund Cat, Calderon Robert H, Wakao Setsuko, Niyogi Krishna K
Abstract excerpt
Chlamydomonas reinhardtii is a unicellular green alga that is a key model organism in the study of photosynthesis and oxidative stress. Here we describe the large-scale generation of a population of insertional mutants that have been screened for phenotypes related to photosynthesis and the isolation of 459 flanking sequence tags from 439 mutants. Recent phylogenomic analysis has identified a core set of genes,...
Topics
- Acetates
- Chlamydomonas reinhardtii
- Mutagenesis, Insertional
- Mutation
- Photosynthesis
- Plant Proteins