Article
A fluorescence-activated cell sorting-based strategy for rapid isolation of high-lipid Chlamydomonas mutants.
The Plant journal : for cell and molecular biology - 1 Jan 2015
Terashima Mia, Freeman Elizabeth S, Jinkerson Robert E, Jonikas Martin C
Abstract excerpt
There is significant interest in farming algae for the direct production of biofuels and valuable lipids. Chlamydomonas reinhardtii is the leading model system for studying lipid metabolism in green algae, but current methods for isolating mutants of this organism with a perturbed lipid content are slow and tedious. Here, we present the Chlamydomonas high-lipid sorting (CHiLiS) strategy, which enables enrichment...
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