Article
Internal amplification control of PCR for the Glu1-Dx5 allele in wheat.
Genetics and molecular research : GMR - 17 Aug 2017
Heim H N, Vieira E S N, Polo L R T, Lima N K, Silva G J, Linde G A, Colauto N B, Schuster I
Abstract excerpt
One of the limiting factors in using dominant markers is the unique amplification of the target fragment. Therefore, failures in polymerase chain reaction (PCR) or non-amplifications can be interpreted as an absence of the allele. The possibility of false negatives implies in reduced efficiency in the selection process in genetic breeding programs besides the loss of valuable genetic material. Thus, this study...
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