Article
Cost-effective duplex Kompetitive Allele Specific PCR markers for homologous genes facilitating wheat breeding.
BMC plant biology - 1 Mar 2023
Jiang Peng, Fan Xiangyun, Zhang Guangxu, Wu Lei, He Yi, Li Chang, Zhang Xu
Abstract excerpt
BACKGROUND: Owing to successful cloning of wheat functional genes in recent years, more traits can be selected by diagnostic markers, and consequently, effective molecular markers will be powerful tools in wheat breeding programs. RESULTS: The present study proposed a cost-effective duplex Kompetitive Allele Specific PCR (dKASP) marker system that combined multiplex PCR and KASP™ technology to yield twice the...
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