Article
"Squirrel" Primer-Based PCR Assay for Direct and Targeted Sanger Sequencing of Short Genomic Segments.
Journal of biomolecular techniques : JBT - 1 Sept 2017
Ebili Henry O, Hassall James C, Fadhil Wakkas, Ham-Karim Hersh, Asiri Abutaleb, Raposo Teresa P, Agboola Ayodeji Johnson, Ilyas Mohammad
Abstract excerpt
Currently, short DNA segments of sub-100 bp can be sequenced either directly by next-generation sequencing and pyrosequencing, which are expensive, or indirectly, via Sanger sequencing combined with the cumbersome and failure-prone plasmid cloning. To circumvent these issues, we have generated a novel sequencing-purposed PCR assay using long-tailed primers (squirrel primers) to Sanger sequence directly sub-100 bp...
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