Article
Cryopreservation protocol for human biliary tree stem/progenitors, hepatic and pancreatic precursors.
Scientific reports - 20 Jul 2017
Nevi Lorenzo, Cardinale Vincenzo, Carpino Guido, Costantini Daniele, Di Matteo Sabina, Cantafora Alfredo, Melandro Fabio, Brunelli Roberto, Bastianelli Carlo, Aliberti Camilla, Monti Marco, Bosco Daniela, Berloco Pasquale Bartolomeo, Panici Pierluigi Benedetti, Reid Lola, Gaudio Eugenio, Alvaro Domenico
Abstract excerpt
Human biliary tree stem/progenitor cells (hBTSCs) are being used for cell therapies of patients with liver cirrhosis. A cryopreservation method was established to optimize sourcing of hBTSCs for these clinical programs and that comprises serum-free Kubota's Medium (KM) supplemented with 10% dimethyl sulfoxide (DMSO), 15% human serum albumin (HSA) and 0.1% hyaluronans. Cryopreserved versus freshly isolated hBTSCs...
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