Article
Rapid Detection of the mt3243A > G Mutation Using Urine Sediment in Elderly Chinese Type 2 Diabetic Patients.
Journal of diabetes research - 1 Jan 2017
Zhang Yinan, Du Xiujuan, Geng Xinqian, Chu Chen, Lu Huijuan, Shen Yixie, Chen Ruihua, Fang Pingyan, Feng Yanmei, Zhang Xiaojie, Chen Yan, Zhou Yanping, Wang Congrong, Jia Weiping
Abstract excerpt
OBJECTIVE: In this study, we aimed to identify mt3243A > G mutation carriers in a group of Chinese elderly type 2 diabetic patients by a rapid and noninvasive diagnostic system. METHODS: DNA was extracted from blood, saliva, and urine sediment samples. The mutation screening and quantitation of heteroplasmy were performed by high-resolution melting (HRM) curve and pyrosequencing, respectively. Patients with...
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