Article
Generation of a Collection of Mutant Tomato Lines Using Pooled CRISPR Libraries.
Plant physiology - 1 Aug 2017
Jacobs Thomas B, Zhang Ning, Patel Dhruv, Martin Gregory B
Abstract excerpt
The high efficiency of clustered regularly interspaced short palindromic repeats (CRISPR)-mediated mutagenesis in plants enables the development of high-throughput mutagenesis strategies. By transforming pooled CRISPR libraries into tomato (Solanum lycopersicum), collections of mutant lines were generated with minimal transformation attempts and in a relatively short period of time. Identification of the targeted...
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