Article
New approach of genetic characterization of group A rotaviruses by the nanopore sequencing method.
Journal of virological methods - 1 Jun 2021
Faizuloev Evgeny, Mintaev Ramil, Petrusha Olga, Marova Anna, Smirnova Daria, Ammour Yulia, Meskina Elena, Sergeev Oleg, Zhavoronok Sergey, Karaulov Alexander, Svitich Oxana, Zverev Vitaly
Abstract excerpt
Nanopore sequencing of virus genomes represented by segmented RNA (e.g. rotaviruses) requires the development of specific approaches. Due to the massive use of rotavirus vaccines, the relevance of monitoring the genetic diversity of circulating strains of group A rotaviruses (RVA) increased. The WHO recommended method of multiplex type-specific PCR does not allow genotyping of all clinically significant strains...
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