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Article

Amplification and next generation sequencing of near full-length human enteroviruses for identification and characterisation from clinical samples.

Scientific reports - 8 Aug 2018

Isaacs Sonia R, Kim Ki Wook, Cheng Junipearl X, Bull Rowena A, Stelzer-Braid Sacha, Luciani Fabio, Rawlinson William D, Craig Maria E

Abstract excerpt

More than 100 different enterovirus (EV) genotypes infect humans and contribute to substantial morbidity. However, current methods for characterisation of full-length genomes are based on Sanger sequencing of short genomic regions, which are labour-intensive and do not enable comprehensive characterisation of viral populations. Here, we describe a simple and sensitive protocol for the amplification and sequencing...

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Identifiers

PMID: 30089864
DOI: 10.1038/s41598-018-30322-y
PMCID: PMC6082906

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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