Article
In vitro biomimetic platforms featuring a perfusion system and 3D spheroid culture promote the construction of tissue-engineered corneal endothelial layers.
Scientific reports - 10 Apr 2017
Li Shanyi, Han Yuting, Lei Hao, Zeng Yingxin, Cui Zekai, Zeng Qiaolang, Zhu Deliang, Lian Ruiling, Zhang Jun, Chen Zhe, Chen Jiansu
Abstract excerpt
Corneal endothelial cells (CECs) are very important for the maintenance of corneal transparency. However, in vitro, CECs display limited proliferation and loss of phenotype via endothelial to mesenchymal transformation (EMT) and cellular senescence. In this study, we demonstrate that continuous supplementary nutrition using a perfusion culture bioreactor and three-dimensional (3D) spheroid culture can be used to...
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