Article
Creating a patient carried Men1 gene point mutation on wild type iPSCs locus mediated by CRISPR/Cas9 and ssODN.
Stem cell research - 1 Jan 2017
Guo Dongsheng, Liu Haikun, Gao Ge, Liu Yanli, Zhuang Yuanqi, Yang Fan, Wang Kepin, Zhou Tiancheng, Qin Dajiang, Hong Liangqing, Li Jialiang, Xu Kecheng, Li Yin-Xiong
Abstract excerpt
A patient specific point mutation (c.1288G>T) of Men1 gene was introduced into wide type iPSC line with CRISPR/Cas9 and single-stranded donor oligonucleotides carrying the mutation. The mutated iPSC line has a heterozygous c.1288G>T mutation on exon-9 of Men1 that was confirmed by sequencing analysis. The karyotype of this line was normal and the pluripotency was demonstrated by its ability to differentiate into...
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