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Article

Generation of WTD, a control human iPSC line for genetic research.

Stem cell research - 1 Dec 2025

Macklin Bria L, Runyon Wendy V, Feliciano Carissa M, Dierks Philip H, Kelly Kaitlin R, Watry Hannah L, Judge Luke M, Conklin Bruce R

Abstract excerpt

The establishment of well characterized control iPSC lines is essential for robust, reproducible research across laboratories. We used CRISPR/Cas9 to derive an isogeneic control line from a patient-derived iPSC line carrying a mutation in the NEFL gene (E396K). After correction of the E396K mutation, UCSFi003-A (WTD) exhibits multi-lineage differentiation potential, a normal karyotype, no large genomic...

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Identifiers

PMID: 41330274
DOI: 10.1016/j.scr.2025.103872
PMCID: PMC13354316

Genes

NEFL

Variants

Provenance

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