Article
A genetic screen implicates a CWC16/Yju2/CCDC130 protein and SMU1 in alternative splicing in Arabidopsis thaliana.
RNA (New York, N.Y.) - 1 Jul 2017
Kanno Tatsuo, Lin Wen-Dar, Fu Jason L, Matzke Antonius J M, Matzke Marjori
Abstract excerpt
To identify regulators of pre-mRNA splicing in plants, we developed a forward genetic screen based on an alternatively spliced GFP reporter gene in Arabidopsis thaliana In wild-type plants, three major splice variants issue from the GFP gene but only one represents a translatable GFP mRNA. Compared to wild-type seedlings, which exhibit an intermediate level of GFP expression, mutants identified in the screen...
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