Article
Patterns of cross-contamination in a multispecies population genomic project: detection, quantification, impact, and solutions.
BMC biology - 29 Mar 2017
Ballenghien Marion, Faivre Nicolas, Galtier Nicolas
Abstract excerpt
BACKGROUND: Contamination is a well-known but often neglected problem in molecular biology. Here, we investigated the prevalence of cross-contamination among 446 samples from 116 distinct species of animals, which were processed in the same laboratory and subjected to subcontracted transcriptome sequencing. RESULTS: Using cytochrome oxidase 1 as a barcode, we identified a minimum of 782 events of between-species...
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