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Article

A plate method for rapid screening of Ketogulonicigenium vulgare mutants for enhanced 2-keto-l-gulonic acid production.

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] - 1 Jan 2000

Yang Weichao, Han Litao, Mandlaa Mandlaa, Zhang Haihong, Zhang Zhongze, Xu Hui

Abstract excerpt

A new plate method was developed for rapid screening of Ketogulonicigenium vulgare mutants overproducing 2-keto-l-gulonic acid (2-KLG). The screening methodology took the advantage of the acidity caused by 2-KLG, which changes the color of bromothymol blue (pH indicator) from blue to yellow. Using the proposed method, a mutant, K. vulgare 65, was selected from 20,000 colonies produced by a strain subjected to...

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Identifiers

PMID: 28292630
DOI: 10.1016/j.bjm.2017.02.002
PMCID: PMC5498444

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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