Article
Relationship between somatic mosaicism of Pax6 mutation and variable developmental eye abnormalities-an analysis of CRISPR genome-edited mouse embryos.
Scientific reports - 3 Mar 2017
Yasue Akihiro, Kono Hitomi, Habuta Munenori, Bando Tetsuya, Sato Keita, Inoue Junji, Oyadomari Seiichi, Noji Sumihare, Tanaka Eiji, Ohuchi Hideyo
Abstract excerpt
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system is a rapid gene-targeting technology that does not require embryonic stem cells. To demonstrate dosage effects of the Pax6 gene on eye formation, we generated Pax6-deficient mice with the CRISPR/Cas system. Eyes of founder embryos at embryonic day (E) 16.5 were examined and categorized according to...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.