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Article

Analysis of amplicon-based NGS data from neurological disease gene panels: a new method for allele drop-out management.

BMC bioinformatics - 8 Nov 2016

Zucca Susanna, Villaraggia Margherita, Gagliardi Stella, Grieco Gaetano Salvatore, Valente Marialuisa, Cereda Cristina, Magni Paolo

Abstract excerpt

BACKGROUND: Amplicon-based targeted resequencing is a commonly adopted solution for next-generation sequencing applications focused on specific genomic regions. The reliability of such approaches rests on the high specificity and deep coverage, although sequencing artifacts attributable to PCR-like amplification can be encountered. Between these artifacts, allele drop-out, which is the preferential amplification...

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Identifiers

PMID: 28185542
DOI: 10.1186/s12859-016-1189-0
PMCID: PMC5123238

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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