Development of a gene panel for next-generation sequencing of clinically relevant mutations in cell-free DNA from cancer patients.

doi:10.1038/bjc.2017.8

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Community

Back to search

Article

Development of a gene panel for next-generation sequencing of clinically relevant mutations in cell-free DNA from cancer patients.

British journal of cancer - 14 Mar 2017

Malapelle Umberto, Mayo de-Las-Casas Clara, Rocco Danilo, Garzon Monica, Pisapia Pasquale, Jordana-Ariza Nuria, Russo Maria, Sgariglia Roberta, De Luca Caterina, Pepe Francesco, Martinez-Bueno Alejandro, Morales-Espinosa Daniela, González-Cao María, Karachaliou Niki, Viteri Ramirez Santiago, Bellevicine Claudio, Molina-Vila Miguel Angel, Rosell Rafael, Troncone Giancarlo

Abstract excerpt

BACKGROUND: When tumour tissue is unavailable, cell-free DNA (cfDNA)can serve as a surrogate for genetic analyses. Because mutated alleles in cfDNA are usually below 1%, next-generation sequencing (NGS)must be narrowed to target only clinically relevant genes. In this proof-of-concept study, we developed a panel to use in ultra-deep sequencing to identify such mutations in cfDNA. METHODS: Our panel ('SiRe')...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Antineoplastic Combined Chemotherapy Protocols

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Carcinoma, Non-Small-Cell Lung

Share to DNA, Neoplasm

Share to Gastrointestinal Neoplasms

Share to Gastrointestinal Stromal Tumors

Share to Melanoma

Share to Neoplasm Staging

Share to ROC Curve

Source

Open in PubMed Open full text

Identifiers

PMID: 28170370
DOI: 10.1038/bjc.2017.8
PMCID: PMC5355934

Genes

EGFR
BRAF
KRAS
NRAS

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Development of a gene panel for next-generation sequencing of clinically relevant mutations in cell-free DNA from cancer patients.PMID 28170370Read ↗

Cell free DNA analysis by SiRe® next generation sequencing panel in non small cell lung cancer patients: focus on basal settingSemantic proximity Read ↗

Performance analysis of SiRe next-generation sequencing panel in diagnostic setting: focus on NSCLC routine samplesSemantic proximity Read ↗

Validity of Targeted Next-Generation Sequencing in Routine Care for Identifying Clinically Relevant Molecular Profiles in Non-Small-Cell Lung Cancer: Results of a 2-Year Experience on 1343 Samples.Semantic proximity Read ↗

Ultra-deep next-generation sequencing of plasma cell-free DNA in patients with advanced lung cancers: results from the Actionable Genome ConsortiumSemantic proximity Read ↗

Prospective blinded study of somatic mutation detection in cell-free DNA utilizing a targeted 54-gene next generation sequencing panel in metastatic solid tumor patients.Semantic proximity Read ↗

Plasma Cell-Free DNA Genotyping: From an Emerging Concept to a Standard-of-Care Tool in Metastatic Non-Small Cell Lung Cancer.Semantic proximity Read ↗

Circulating Cell-Free Tumor DNA Analysis of 50 Genes by Next-Generation Sequencing in the Prospective MOSCATO Trial.Semantic proximity Read ↗

Molecular Analysis of Circulating Cell-Free DNA from Lung Cancer Patients in Routine Laboratory Practice: A Cross-Platform Comparison of Three Different Molecular Methods for Mutation Detection.Semantic proximity Read ↗

Ion Torrent next-generation sequencing for routine identification of clinically relevant mutations in colorectal cancer patients.Semantic proximity Read ↗

The relevance of the reference range for EGFR testing in non-small cell lung cancer patients.Semantic proximity Read ↗

Clinical validation of a next-generation sequencing assay for 17 cancer related mutations in non-small cell Lung cancerSemantic proximity Read ↗

Cell-Free DNA Next-Generation Sequencing Prediction of Response and Resistance to Third-Generation EGFR InhibitorSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.