Article
Phosphorylated fraction of H2AX as a measurement for DNA damage in cancer cells and potential applications of a novel assay.
PloS one - 1 Jan 2017
Ji Jiuping, Zhang Yiping, Redon Christophe E, Reinhold William C, Chen Alice P, Fogli Laura K, Holbeck Susan L, Parchment Ralph E, Hollingshead Melinda, Tomaszewski Joseph E, Dudon Quentin, Pommier Yves, Doroshow James H, Bonner William M
Abstract excerpt
Phosphorylated H2AX (γ-H2AX) is a sensitive marker for DNA double-strand breaks (DSBs), but the variability of H2AX expression in different cell and tissue types makes it difficult to interpret the meaning of the γ-H2AX level. Furthermore, the assays commonly used for γ-H2AX detection utilize laborious and low-throughput microscopy-based methods. We describe here an ELISA assay that measures both phosphorylated...
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