Article
Quantification of Plasmid Copy Number with Single Colour Droplet Digital PCR.
PloS one - 1 Jan 2017
Plotka Magdalena, Wozniak Mateusz, Kaczorowski Tadeusz
Abstract excerpt
Bacteria can be considered as biological nanofactories that manufacture a cornucopia of bioproducts most notably recombinant proteins. As such, they must perfectly match with appropriate plasmid vectors to ensure successful overexpression of target genes. Among many parameters that correlate positively with protein productivity plasmid copy number plays pivotal role. Therefore, development of new and more...
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