Article
Utility of Systematic Isolation of immune cell subsets from HIV-infected individuals for miRNA profiling.
Journal of immunological methods - 1 Mar 2017
Bargalló Manel E, Guardo Alberto C, Maleno Maria J, Miralles Laia, Egaña-Gorroño Lander, Escribà Tuixent, García Felipe, Gatell Jose M, Arnedo Mireia, Plana Montserrat
Abstract excerpt
INTRODUCTION: Peripheral blood mononuclear cells (PBMCs) are frequently used for genomic analyses, but several factors can affect the yield and integrity of nucleic acids, including the methods of cell collection and isolation. The goal of this work was to analyze the utility of systematic isolation of different immune cell subsets by immunomagnetic separation and the RNA integrity after isolated cells from...
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