Article

Combined protein and transcript single cell RNA sequencing in human peripheral blood mononuclear cells

2020-09-12

Abstract excerpt

Cryopreserved peripheral blood mononuclear cells (PBMCs) are frequently collected and provide disease- and treatment-relevant data in clinical studies. Here, we developed combined protein (40 antibodies) and transcript single cell (sc)RNA sequencing in PBMCs. Among 31 participants in the WIHS Study, we sequenced 41,611 cells. Using Boolean gating followed by Seurat UMAPs and Louvain clustering, we identified 58 su...

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Literature Corpus work: 5e4d1a28-745e-555e-8769-27347d450130
DOI: 10.1101/2020.09.10.292086

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