Article
Evolutionary drivers of thermoadaptation in enzyme catalysis.
Science (New York, N.Y.) - 20 Jan 2017
Nguyen Vy, Wilson Christopher, Hoemberger Marc, Stiller John B, Agafonov Roman V, Kutter Steffen, English Justin, Theobald Douglas L, Kern Dorothee
Abstract excerpt
With early life likely to have existed in a hot environment, enzymes had to cope with an inherent drop in catalytic speed caused by lowered temperature. Here we characterize the molecular mechanisms underlying thermoadaptation of enzyme catalysis in adenylate kinase using ancestral sequence reconstruction spanning 3 billion years of evolution. We show that evolution solved the enzyme's key kinetic obstacle-how to...
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