Article
Reconstructing the plant mitochondrial genome for marker discovery: a case study using Pinus.
Molecular ecology resources - 1 Sept 2017
Donnelly Kevin, Cottrell Joan, Ennos Richard A, Vendramin Giovanni Giuseppe, A'Hara Stuart, King Sarah, Perry Annika, Wachowiak Witold, Cavers Stephen
Abstract excerpt
Whole-genome-shotgun (WGS) sequencing of total genomic DNA was used to recover ~1 Mbp of novel mitochondrial (mtDNA) sequence from Pinus sylvestris (L.) and three members of the closely related Pinus mugo species complex. DNA was extracted from megagametophyte tissue from six mother trees from locations across Europe, and 100-bp paired-end sequencing was performed on the Illumina HiSeq platform. Candidate mtDNA...
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