Article
Comparison among three methods for obtaining chloroplast genome sequences from the conifer Pinus massoniana.
Genomics - 1 May 2020
Ni ZhouXian, Ye YouJu, Xu Meng, Xu Li-An
Abstract excerpt
The chloroplast genome (CPG) is a powerful tool for phylogenetic studies. Many CPGs have been determined using NGS. However, the large nuclear-genome and difficult CPG-DNA separation in conifers limit their application in related research. In this study, three methods (PCR + Sanger, PCR + HiSeq, cpDNA+HiSeq) for obtaining the CPGs of Pinus massoniana were compared for sequence accuracy, time and cost....
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