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Article

Systematic characterization of artificial small RNA-mediated inhibition of Escherichia coli growth.

RNA biology - 1 Feb 2017

Noro Emiko, Mori Masaru, Makino Gakuto, Takai Yuki, Ohnuma Sumiko, Sato Asako, Tomita Masaru, Nakahigashi Kenji, Kanai Akio

Abstract excerpt

A new screening system for artificial small RNAs (sRNAs) that inhibit the growth of Escherichia coli was constructed. In this system, we used a plasmid library to express RNAs of ∼120 nucleotides, each with a random 30-nucleotide sequence that can recognize its target mRNA(s). After approximately 60,000 independent colonies were screened, several plasmids that inhibited bacterial growth were isolated. To...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 27981881
DOI: 10.1080/15476286.2016.1270001
PMCID: PMC5324740

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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